Anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal Antibody

Product Name

Anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal Antibody

View all B7-2/CD86 Antibodies

SKU/Catalog Number

M00220

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal Antibody (Catalog # M00220). Tested in Functional Assay, Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00220)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: BU63

Isotype

IgG1, kappa

Immunogen

ARH-77 (B-lymphoblastoid cell line)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00220 is reactive to CD86 in Human, Mouse, Rat

Applications

M00220 is guaranteed for Flow Cytometry, IF, IHC, Functional Assay Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

37.682kDa

Background of B7-2/CD86

Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Functional Studies (Order Ab without Azide)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Flow Cytometric Analysis of human PBMCs using Anti-CD86 Mouse Monoclonal Antibody (BU63); Goat anti-Mouse IgG-CF488 (red); Isotype Control (green).

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Formalin-fixed, paraffin-embedded human Esophageal Tumor stained with Anti-CD86 Mouse Monoclonal Antibody (BU63).

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Immunofluorescence staining of PFA-fixed Ramos cells using Anti-CD86 Mouse Monoclonal Antibody (BU63) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

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Flow Cytometric analysis of PFA-fixed Ramos cells. Anti-CD86 Mouse Monoclonal Antibody (BU63) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Gene/Protein Information For CD86 (Source: Uniprot.org, NCBI)

Alternative Names

Activation B7-2 antigen; B70; B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD28LG2B7-2 antigen); CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86 CD86 B7-2, B7.2, B70, CD28LG2, LAB72 CD86 molecule T-lymphocyte activation antigen CD86|B-lymphocyte activation antigen B7-2|BU63|CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)|CTLA-4 counter-receptor B7.2|FUN-1

For more info on CD86, check out the CD86 Infographic

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In this infographic, you will see the following information for CD86: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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